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JoVE Journal Cancer Research
Análise digital de imunocoloração da proteína ZW10 interagindo em tecidos pulmonares humanos
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Análise digital de imunocoloração da proteína ZW10 interagindo em tecidos pulmonares humanos

Article DOI: 10.3791/58551-v • 07:40 min • May 1st, 2019
May 1st, 2019 •
1, 2, 3, 1, 4, 4, 5, 6, 4, 4, 4, 1
1Department of Hematology, Zhongnan Hospital of Wuhan University, 2Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 3Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 4Department of Immunology, School of Basic Medical Sciences, Wuhan University, 5Department of Gastroenterology, Central Hospital of Wuhan, 6Department of Transfusion, Zhongnan Hospital of Wuhan University

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A proteína de interação ZW10 (ZWINT) participa no ponto de verificação mitotic do eixo e na patogénese da carcinoma. Aqui, apresentamos uma metodologia de imunocoloração de ZWINT em tecidos de câncer de pulmão humano, seguido pela digitalização digital de slides inteiros e análise de imagem. Esta metodologia pode fornecer imagens digitais de alta qualidade e resultados confiáveis.

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Pesquisa do cancro edição 147 técnica digital automática cancro de pulmão ZW10 que interage a proteína immunocorante imagem latente inteira-corrediça digitalizada H-contagem
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