Immunology and Infection
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Génie de génome CRISPR dotés de Cas9 pour produire des modèles de Jurkat Reporter pour l’Infection à VIH-1 avec les Sites d’intégration Proviral sélectionnés
Chapters
Summary
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Nous présentons un flux de travail de génie du génome pour la génération de nouveaux modèles in vitro pour l’infection à VIH-1 qui récapitulent l’intégration provirale dans certains sites génomiques. Ciblage des journalistes dérivé du VIH est facilitée par la manipulation du génome CRISPR Cas9-médiation, in situ. Des protocoles détaillés pour la génération de cellule unique clone, dépistage et vérification de ciblage correcte sont fournis.
Tags
Immunologie et Infection numéro 141 le VIH la latence du VIH de sites d’intégration du VIH de modèle de culture cellulaire ingénierie du génome CRISPR-Cas9Related Videos
PLAYLIST
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Research • BiologyGeneration of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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Research • GeneticsCRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
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Research • GeneticsCell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Research • BioengineeringCRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
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Research • GeneticsCRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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Research • Cancer ResearchUsing CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
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Research • Cancer ResearchVirus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
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Research • Cancer ResearchInvestigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
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Research • Cancer ResearchUsing CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
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Research • GeneticsInvestigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Research • GeneticsHighly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
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Research • Cancer ResearchProstate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses
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Research • Immunology and InfectionCRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites
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Research • GeneticsA Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Research • GeneticsAmplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
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Research • Immunology and InfectionEvaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
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Research • GeneticsUsing a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Research • GeneticsIntroducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
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Research • GeneticsOverexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Research • GeneticsEmbryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus
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Research • Immunology and InfectionLentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
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Research • Immunology and InfectionGenerating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
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Research • GeneticsUse of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
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Research • BioengineeringProduction of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
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Research • BiologySilencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
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Research • GeneticsCRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
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Research • BiologyAgrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
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Research • BiologyHigh-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
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Research • GeneticsTransforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
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Research • GeneticsA Robotic Platform for High-throughput Protoplast Isolation and Transformation
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Research • BioengineeringMicroinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
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Research • GeneticsEndogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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Research • Developmental BiologyEstablishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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Research • Developmental BiologyGenerating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
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Research • Developmental BiologyCRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors
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Research • GeneticsIntroducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing