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JoVE Journal
Bioengineering

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多夏巴氏杆菌自主表达采用除抑制技术
 
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多夏巴氏杆菌自主表达采用除抑制技术

Article doi: 10.3791/58589
January 23rd, 2019

Summary January 23rd, 2019

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Click here for the English version.

该方法首次介绍了在毕赤酵母 (komagataella phaffii)高效诱导无甲醇重组蛋白生产的可靠实验程序, 并采用了碳源调节启动子 p dc和 pdf 在小规模试验中, 在可在线监测培养参数的同时, 可采用恒定的甘油饲料。

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