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Kvasi-metagenomic analyse af Salmonella fra mad og miljøprøver

Article DOI: 10.3791/58612-v • 06:12 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Center for Food Safety

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Vi præsenterer her, en protokol for at forberede samordnet registrerings- og subtyping af Salmonella gennem quasimetagenomic sekventering DNA-prøver fra mad og miljø microbiomes. Den kombinerede brug af kultur berigelse, immunomagnetic adskillelse (IMS) og flere forskydning forstærkning (MDA) giver mulighed for effektiv koncentration af Salmonella genomisk DNA fra mad og miljøprøver.

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Biologi spørgsmål 140 quasimetagenomics immunomagnetic adskillelse (IMS) samlede genom forstærkning sekventering opdagelse subtyping Salmonella
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