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Isolering av mus epidermal keratinocyter och deras in vitro Clonogenic kultur
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Isolering av mus epidermal keratinocyter och deras in vitro Clonogenic kultur

Article DOI: 10.3791/58701-v • 06:16 min • August 10th, 2019
August 10th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Hormel Institute, University of Minnesota

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Målet med detta protokoll är att isolera epidermal keratinocyter från den dorsala huden hos vuxna möss för en mängd olika nedströms tillämpningar såsom molekylärbiologi, biokemi, fluorescens aktiverad cell sortering, och primära in vitro-användning (t. ex., klonogena keratinocyter).

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Biologi epidermal keratinocyter cellkultur vuxen mus epidermis klonala kulturen keratinocyter stamceller 3T3 fibroblast
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