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Biochemistry

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JoVE Journal Biochemistry
Snabb fluorescens-baserade karakterisering av enda extracellulär blåsor i människans blod med nanopartiklar-tracking analys
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Snabb fluorescens-baserade karakterisering av enda extracellulär blåsor i människans blod med nanopartiklar-tracking analys

Article DOI: 10.3791/58731-v • 09:16 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1
1Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University

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I detta protokoll beskriver vi komplett arbetsflöde för snabb isolering av extracellulära blåsor från humant helblod och karakterisering av specifika markörer genom fluorescens-baserade nanopartikel-tracking-analys. De presenterade resultaten visar en hög reproducerbarhet och kan justeras till cell cellkulturer.

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Fråga 143 nanovesicles Exosomes biokemi partikel spårning analys CD63 CD9 vimentin serum
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