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Identifikation af funktionelle Protein regioner gennem kimære Protein konstruktion
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Identification of Functional Protein Regions Through Chimeric Protein Construction
DOI:

11:39 min

January 08, 2019

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Chapters

  • 00:04Title
  • 00:49Chimeric Protein Design
  • 05:00Preparation for Molecular Cloning
  • 07:05Polymerase Chain Reaction (PCR) Amplification of the Individual DNA Fragments Forming the Chimera and to Generate the Chimeric DNA Sequence
  • 09:22Results: Generation of the Oncostatin M BC Loop Chimera
  • 10:55Conclusion

Summary

Automatic Translation

Strukturelt relaterede proteiner udøve ofte forskellige biologiske funktioner. Udveksling af tilsvarende regioner af disse proteiner for at skabe kimære proteiner udgør en innovativ tilgang til at identificere kritiske protein regioner, der er ansvarlige for deres funktionelle divergens.

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