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Journal / Biochemistry / Festlegung der funktionellen Protein Regionen…
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Biochemistry

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JoVE Journal Biochemistry
Festlegung der funktionellen Protein Regionen durch chimäres Protein Aufbau
Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
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Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Journal (Biochemistry)
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Festlegung der funktionellen Protein Regionen durch chimäres Protein Aufbau

Article DOI: 10.3791/58786-v • 11:39 min • January 8th, 2019
January 8th, 2019 •
1, 1,2, 1,2, 1,2
1Department of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, 2Partner site Rhein-Main, German Centre for Cardiovascular Research (DZHK)

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Strukturell verwandte Proteine üben häufig unterschiedliche biologische Funktionen. Der Austausch von äquivalenten Regionen dieser Proteine um Chimären Proteinen zu schaffen stellt einen innovativen Ansatz zur kritischen Protein Regionen zu identifizieren, die für ihre funktionale Divergenz verantwortlich sind.

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Biochemie Ausgabe 143 Chimeric Proteine strukturelle Ähnlichkeit Molekularbiologie überlappen PCR Struktur-Funktions-Analyse Domänen Protein Protein-Protein-Wechselwirkungen Protein Bindungseigenschaften
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