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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

DOI:
10.3791/58821-v

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10:49 min

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January 28, 2019

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,
1Department of Civil and Environmental Engineering,Tohoku University, 2Department of Frontier Science for Advanced Environment, Graduate School of Environmental Studies,Tohoku University

Chapters

  • 00:04Title
  • 00:42Cell Culture
  • 02:20Specific Growth Rate of Rotavirus
  • 06:12Cell-binding Assay
  • 08:53Results: Growth Curve of Rotavirus and Cell-binding Assay of Five Rhesus Rotavirus (RRV) Strains Purified from Plaques
  • 09:59Conclusion

Summary

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Here we present two protocols, one for measuring the specific growth rate and the other for the cell-binding ability of rotavirus using the plaque assay and RT-qPCR. These protocols are available for confirming the differences in phenotypes between rotavirus strains.

Tags

Keywords: RotavirusPlaque AssayCell-binding AssayMA104 Cell LineCell CultureVirus TiterDisinfectant-resistant StrainsPhenotypic ChangeVirus PropagationVirus ActivationMultiplicity Of Infection (MOI)

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