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Journal
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Immunology and Infection
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轮状病毒的特定生长速率及细胞结合能力的测定
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Please note that all translations are automatically generated.
Click here for the English version.
轮状病毒的特定生长速率及细胞结合能力的测定
DOI:
10.3791/58821-v
•
10:49 min
•
January 28, 2019
•
Syun-suke Kadoya
,
Daisuke Sano
2
1
Department of Civil and Environmental Engineering
,
Tohoku University
,
2
Department of Frontier Science for Advanced Environment, Graduate School of Environmental Studies
,
Tohoku University
Chapters
00:04
Title
00:42
Cell Culture
02:20
Specific Growth Rate of Rotavirus
06:12
Cell-binding Assay
08:53
Results: Growth Curve of Rotavirus and Cell-binding Assay of Five Rhesus Rotavirus (RRV) Strains Purified from Plaques
09:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了两个方案, 一个用于测量特定的生长速率, 另一个是利用斑块分析和 rt-qpcr 来测量轮状病毒的细胞结合能力。这些协议可用于确认轮状病毒株之间表型的差异。
Tags
Rotavirus
Plaque Assay
Cell-binding Assay
MA104 Cell Line
Cell Culture
Virus Titer
Disinfectant-resistant Strains
Phenotypic Change
Virus Propagation
Virus Activation
Multiplicity Of Infection (MOI)
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