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Journal / Chemistry / Preparação de Poly(pentafluorophenyl acrylate)…
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JoVE Journal Chemistry
Preparação de Poly(pentafluorophenyl acrylate) acrescida SiO2 grânulos para purificação de proteínas
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Preparação de Poly(pentafluorophenyl acrylate) acrescida SiO2 grânulos para purificação de proteínas

Article DOI: 10.3791/58843-v • 08:51 min • November 19th, 2018
November 19th, 2018 •
1, 1,2, 1, 1,2, 1
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 2KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

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Um protocolo para a preparação de poli (acrilato de pentafluorophenyl) (poly(PFPA)) enxertados grânulos de sílica é apresentado. A superfície funcionalizados poly(PFPA) é então imobilizada com anticorpos e usada com sucesso para a separação de proteínas através da imunoprecipitação.

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Química edição 141 poli (acrilato de pentafluorophenyl) 3-aminopropyltriethoxysilane escova de polímero reativo pós-polimerização functionalization imobilização do anticorpo imunoprecipitação
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