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Beredning av Poly(pentafluorophenyl acrylate) Functionalized SiO2 pärlor för Protein rening
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
DOI:

08:51 min

November 19, 2018

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Chapters

  • 00:04Title
  • 00:54Preparation of Poly(PFPA) Functionalized SiO2 Beads
  • 02:31Preparation of SiO2 Beads Grafted with PEG-Substituted Poly(PFPA)
  • 04:06Antibody Immobilization on Poly(PFPA) Grafted SiO2 Beads
  • 05:20Cell Lysis and Immunoprecipitation
  • 07:00Results: Analysis of Poly (pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
  • 08:09Conclusion

Summary

Automatic Translation

Ett protokoll för beredning av poly (pentafluorophenyl akrylat) (poly(PFPA)) ympade kiseldioxid pärlor presenteras. Poly(PFPA) functionalized ytan sedan orörlig med antikroppar och används framgångsrikt för protein separation genom immunoprecipitation.

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