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减法基因组学的新序列发现
JoVE Journal
Genetics
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JoVE Journal Genetics
Novel Sequence Discovery by Subtractive Genomics
DOI:

09:40 min

January 25, 2019

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Chapters

  • 00:04Title
  • 01:08De novo Assemble Starting Sequence
  • 02:03BLAST the Assembly Against the Reference Sequence
  • 04:44Design Primers for the Sequence that Remains
  • 06:25qPCR Validation of the Remaining Sequence
  • 07:51Results: Example Data of qPCR Results Including Negative and Positive Outcomes
  • 08:53Conclusion

Summary

Automatic Translation

该协议的目的是使用计算和工作台研究的组合来寻找无法轻易从共同净化序列中分离的新序列, 这可能只是部分已知的序列。

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