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Journal
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Genetics
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减法基因组学的新序列发现
JoVE Journal
Genetics
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JoVE Journal
Genetics
Novel Sequence Discovery by Subtractive Genomics
Please note that all translations are automatically generated.
Click here for the English version.
减法基因组学的新序列发现
DOI:
10.3791/58877-v
•
09:40 min
•
January 25, 2019
•
Kathryn C. Asalone
,
Megan M. Nelson
,
John R. Bracht
1
Biology Department
,
American University
Chapters
00:04
Title
01:08
De novo Assemble Starting Sequence
02:03
BLAST the Assembly Against the Reference Sequence
04:44
Design Primers for the Sequence that Remains
06:25
qPCR Validation of the Remaining Sequence
07:51
Results: Example Data of qPCR Results Including Negative and Positive Outcomes
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目的是使用计算和工作台研究的组合来寻找无法轻易从共同净化序列中分离的新序列, 这可能只是部分已知的序列。
Tags
Novel Sequence Discovery
Subtractive Genomics
Genomic Sequences
Inexpensive
Flexible
Bacterial Vaccine Targets
Virus Identification
Unknown Separation
Reference Sequence
Trimmomatic
Pear
Reptile
Trinity
Blast
Transdeckor
Tblastn
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