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Journal / Bioengineering / 路西法黄 -…
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Bioengineering

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JoVE Journal Bioengineering
路西法黄 - 人类血脑屏障细胞模型中一个健壮的细胞渗透标记
Fabrication and Characterization of a Conformal Skin-like Electronic System for Quantitative, Cutaneous Wound Management
Journal (Bioengineering)
Fabrication and Characterization of a Conformal Skin-like Electronic System for Quantitative, Cutaneous Wound Management
Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
Journal (Bioengineering)
Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
 
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路西法黄 - 人类血脑屏障细胞模型中一个健壮的细胞渗透标记

Article DOI: 10.3791/58900-v • 06:22 min • August 19th, 2019
August 19th, 2019 •
1, 1, 2, 1
1Department of Pharmaceutical, Administrative and Social Sciences, Duquesne University, 2Department of Pharmaceutical Sciences, University of Kentucky

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我们提出了荧光测定，以证明Lucifer黄色（LY）是一个强大的标记，以确定hCMEC/D3细胞单层的明显准细胞渗透性，人类血脑屏障的体外模型。我们使用这种测定来确定培养的hCMEC/D3细胞中汇体单层形成的动力学。

Tags

生物工程，第150期，荧光素黄色，副细胞渗透性，血脑屏障，hCMEC/D3，DNA纳米粒子，转染。
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