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Journal / Bioengineering / 在大鼠模型中使用低强度聚焦超声破坏血脊髓屏障…
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Bioengineering

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JoVE Journal Bioengineering
在大鼠模型中使用低强度聚焦超声破坏血脊髓屏障
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在大鼠模型中使用低强度聚焦超声破坏血脊髓屏障

Article DOI: 10.3791/65113-v • 09:03 min • March 10th, 2023
March 10th, 2023 •
1, 1,2,3, 1, 1,3, 1, 1, 1, 1, 1,2,3, 1,3, 2, 1, 1,3, 1,2,3,4,5,6
1Department of Neurosurgery, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, Johns Hopkins University, 3HEPIUS Innovation Laboratory, Johns Hopkins University School of Medicine, 4Department of Electrical Engineering and Computer Science, Johns Hopkins University, 5Department of Mechanical Engineering, Johns Hopkins University, 6Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University

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通过静脉注射微泡和应用低强度聚焦超声 （LIFU），可以成功实现血脊髓屏障 （BSCB） 的破坏。该协议详细介绍了在啮齿动物模型中使用LIFU打开BSCB，包括设备设置，微气泡注入，目标定位和BSCB破坏可视化。

Tags

血脊髓屏障破坏、低强度聚焦超声、LIFU、可逆神经调节技术、BSCB破坏法、大鼠模型、动物制备、微泡给药、靶标选择和定位、BSCB破坏可视化和确认、高性价比方法、聚焦超声换能器、超声处理参数评估、脊髓给药、免疫调节、神经调控
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