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Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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Chapters
- 00:04Introduction
- 01:06Antibody Panel Preparation and Antibody Staining
- 02:35Image Acquisition
- 04:31Results: Isotopic Purity and Channel Cross-Talk, Staining Images in Different Tissues, and Images of the Staining Before and After Image Preparation
- 05:59Conclusion
In the era of cancer immunotherapy, interest in elucidating tumor microenvironment dynamics has increased strikingly. This protocol details a mass spectrometry imaging technique with respect to its staining and imaging steps, which allow for highly multiplexed spatial analysis.
