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Genetics
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利用体外和细胞内形状研究小分子诱导的预 mrna 结构变化
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JoVE Journal
Genetics
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
Please note that all translations are automatically generated.
Click here for the English version.
利用体外和细胞内形状研究小分子诱导的预 mrna 结构变化
DOI:
10.3791/59021-v
•
11:58 min
•
January 30, 2019
•
Jingxin Wang
,
John Hammond
,
Kristen A. Johnson
1
Calibr
,
The Scripps Research Institute
,
2
Department of Integrative Structural and Computational Biology
,
The Scripps Research Institute
Chapters
00:04
Title
00:31
32
P-labeled Primer Labeling for In Vitro Selective 2‘-hydroxyl Acylation Analyzed by Primer Extension (SHAPE)
03:44
Small Molecule Binding and RNA 2‘-OH Modification
06:29
Marker Preparation and Primer Extension
08:59
PAGE Analysis
10:15
Results of In Vitro and In-cell SHAPE for Analyzing Small Molecule Induced Pre-mRNA Structural Changes
11:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
通过引物扩展 (shape) 实验分析的体外和细胞内选择性 2 '-羟基酰化的详细方案, 以确定在存在 rna 靶向小分子的情况下, mrna 前序列的二级结构。本文中介绍。
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In Vitro SHAPE
In-cell SHAPE
RNA Secondary Structure
Small Molecule
Pre-mRNA
Reverse Transcription
Radioactive Labeling
Acrylamide Gel
Passive Elution
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