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Chapters
- 00:04Title
- 00:3132P-labeled Primer Labeling for In Vitro Selective 2‘-hydroxyl Acylation Analyzed by Primer Extension (SHAPE)
- 03:44Small Molecule Binding and RNA 2‘-OH Modification
- 06:29Marker Preparation and Primer Extension
- 08:59PAGE Analysis
- 10:15Results of In Vitro and In-cell SHAPE for Analyzing Small Molecule Induced Pre-mRNA Structural Changes
- 11:02Conclusion
Detaljerede protokoller for både in vitro og i celle selektiv 2'-hydroxyl acylation analyseret af primer forlængelse (SHAPE) forsøg at bestemme sekundær struktur af pre-mRNA sekvenser af interesse i overværelse af et RNA-targeting lille molekyle er præsenteret i denne artikel.
