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Ved hjælp af in Vitro og i celler form at undersøge lille molekyle induceret Pre-mRNA strukturelle ændringer
JoVE Journal
Genetics
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JoVE Journal Genetics
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
DOI:

11:58 min

January 30, 2019

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Chapters

  • 00:04Title
  • 00:3132P-labeled Primer Labeling for In Vitro Selective 2‘-hydroxyl Acylation Analyzed by Primer Extension (SHAPE)
  • 03:44Small Molecule Binding and RNA 2‘-OH Modification
  • 06:29Marker Preparation and Primer Extension
  • 08:59PAGE Analysis
  • 10:15Results of In Vitro and In-cell SHAPE for Analyzing Small Molecule Induced Pre-mRNA Structural Changes
  • 11:02Conclusion

Summary

Automatic Translation

Detaljerede protokoller for både in vitro og i celle selektiv 2'-hydroxyl acylation analyseret af primer forlængelse (SHAPE) forsøg at bestemme sekundær struktur af pre-mRNA sekvenser af interesse i overværelse af et RNA-targeting lille molekyle er præsenteret i denne artikel.

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