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Sintesi e Bioconjugation di tiolo-reattive reagenti per la creazione del sito-selettivamente per volta efattori
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Sintesi e Bioconjugation di tiolo-reattive reagenti per la creazione del sito-selettivamente per volta efattori

Article DOI: 10.3791/59063-v • 08:47 min • March 6th, 2019
March 6th, 2019 •
1, 1,2, 1, 1,2,3,4
1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

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In questo protocollo, descriveremo la sintesi di cialde, un reagente basati su solfone metil phenyoxadiazolyl per il fissaggio dei carichi sito selettivo i tioli di biomolecole, in particolare gli anticorpi. Inoltre, descriveremo la sintesi e la caratterizzazione di un chelante bifunzionale baccelli-cuscinetto e la sua coniugazione di un anticorpo di modello.

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Chimica problema 145 bioconjugation site-specific bioconjugation sito-selettivo maleimide tiolo solfidrilico radioimmunoconjugate immunoconjugate
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