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Modifica di genoma in linee cellulari di mammifero usando CRISPR-Cas
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Modifica di genoma in linee cellulari di mammifero usando CRISPR-Cas

Article DOI: 10.3791/59086-v • 07:56 min • April 11th, 2019
April 11th, 2019 •
1, 1, 1,2, 1,2
1Genome Institute of Singapore, Agency for Science Technology and Research, 2School of Chemical and Biomedical Engineering, Nanyang Technological University

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CRISPR-Cas è una potente tecnologia per progettare i complessi genomi delle piante e degli animali. Qui, dettagliamo un protocollo efficiente modificare il genoma umano usando diverse endonucleasi di Cas. Evidenziamo considerazioni importanti e parametri di progettazione per ottimizzare l'efficienza di editing.

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Genetica problema 146 CRISPR Cas9 Cas12a editing genomico unirsi non-omologo fine riparazione omologia-regia
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