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Eksempel forberedelse til masse-massespektrometri-baserte Proteomikk analyse av okulære Microvessels

Article DOI: 10.3791/59140-v • 11:32 min • February 22nd, 2019
February 22nd, 2019 •
*1, 1, 1, 1, 1, 1, *1
1Department of Ophthalmology, University Medical Centre of the Johannes Gutenberg University Mainz
* These authors contributed equally

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Proteom karakteristikk av okulære mikrovaskulær senger er avgjørende for grundig forståelse av mange okulær patologi hos mennesker. Denne studien viser en effektiv, rask og robust metode for protein utvinning og prøve forberedelse fra små blodkar ansette svin kort bakre ciliary arteriene som modell fartøy for masse-massespektrometri-baserte Proteomikk analyser.

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Biologi problemet 144 blodkar microvessels kort bakre ciliary arteriene Proteomikk massespektrometri øye gris
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