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Encellet proteomikkfremstilling for massespektrometrianalyse ved bruk av frysevarmelyse og en isobarisk bærer
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Encellet proteomikkfremstilling for massespektrometrianalyse ved bruk av frysevarmelyse og en isobarisk bærer

Article DOI: 10.3791/63802-v • 06:13 min • December 9th, 2022
December 9th, 2022 •
*1, 1,2,3, *1
1Department of Bioengineering, Northeastern University, 2Barnett Institute, Northeastern University, 3Department of Biology, Northeastern University
* These authors contributed equally

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I denne protokollen beskriver vi hvordan man kan forberede pattedyrceller for encellet proteomikkanalyse via massespektrometri ved bruk av kommersielt tilgjengelige reagenser og utstyr, med muligheter for både manuell og automatisk pipettering.

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