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基于性光谱的眼微血管蛋白质组学样品制备
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Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
Please note that all translations are automatically generated.
Click here for the English version.
基于性光谱的眼微血管蛋白质组学样品制备
DOI:
10.3791/59140-v
•
11:32 min
•
February 22, 2019
•
Natarajan Perumal*
1
,
Lars Straßburger
,
Carsten Schmelter
,
Adrian Gericke
,
Norbert Pfeiffer
,
Franz H. Grus
,
Caroline Manicam*
1
1
Department of Ophthalmology
,
University Medical Centre of the Johannes Gutenberg University Mainz
Chapters
00:04
Title
01:02
Isolation of Short Posterior Ciliary Arteries
02:17
Sample Preparation
03:43
Pellet Digestion
04:40
Sample Cleaning and Buffer Exchange
05:40
One-dimensional Gel Electrophoresis (1DE)
06:50
In-gel Tryptic Digestion
09:23
Results: Comparison of Protein Extraction Efficacy and Robustness and Representative 1DE Gel Analysis
10:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
眼微血管床的蛋白质组特征是深入了解人类许多眼部病理的关键。本研究展示了一种有效、快速、稳健的方法, 用于利用猪短的后睫毛动脉作为基于质谱的蛋白质组学分析的模型血管, 从小血管中提取蛋白质和制备样品。
Tags
Mass Spectrometry
Proteomics
Ocular Microvasculature
Sample Preparation
Dissection
Short Posterior Ciliary Artery
Protein Extraction
Zirconium Oxide Beads
Homogenization
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