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組み立て済みプラスチックマイクロ流体チップ内のヒト幹細胞由来ニューロンの区分化
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Article
DOI:
10.3791/59250-v
•
06:46 min
•
May 3rd, 2019
組み立て済みプラスチックマイクロ流体チップ内のヒト幹細胞由来ニューロンの区分化
May 3rd, 2019
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Smita R. Paranjape1,2, Tharkika Nagendran1,2, Valerie Poole3, Joseph Harris3, Anne Marion Taylor1,2,3
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
このプロトコルは、コンパートメント化されたマイクロ流体チップの使用を示し、環状オレフィン共重合体で成形された注射をヒト幹細胞から分化した培養ニューロンに対して行う。これらのチップは、従来のコンパートメント化ポリ(ジメチルシロキサン)デバイスよりも組み立て済みで使いやすいです。ウイルス標識、流体単離、軸除染、免疫染色など、複数の一般的な実験パラダイムがここに記載されています。
Tags
神経科学 問題 147 マイクロ流体室 環状オレフィン共重合体 ニューロンデバイス マイクロ流体チップ 培養ニューロン マルチコンパートメントチップ ヒト幹細胞由来ニューロン 神経幹細胞 H9幹細胞株 チップ アクソン分離Related Videos
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