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Prøveforberedelsemetode for skanning og overføring elektron mikroskop for vedlegg av treboring beetle

Article DOI: 10.3791/59251-v • 10:09 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 3, 1, 4, 1
1The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, 2School of Forestry, Inner Mongolia Agricultural University, 3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 4School of Forestry, Fujian Agriculture and Forestry University

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For å observere ultrastruktur av insektssensilla, skanning og overføring elektron mikroskopi (SEM og TEM, henholdsvis) prøveforberedelse protokoll ble presentert i studien. Tween 20 ble lagt inn i fikserende for å unngå prøvedeformasjon i SEM. Fluorescence mikroskopi var nyttig for å forbedre kutting nøyaktighet i TEM.

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Miljøvitenskap Utgave 156 insekt olfactory gustatory sensilla ultrastruktur skanning elektronmikroskop transmisjonelektronmikroskop fluorescensmikroskop kjemisk økologi
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