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Journal / Neuroscience / Purificazione della Frazione ricca di Filopodia…
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JoVE Journal Neuroscience
Purificazione della Frazione ricca di Filopodia Dendritica
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Purificazione della Frazione ricca di Filopodia Dendritica

Article DOI: 10.3791/59292-v • 11:51 min • May 2nd, 2019
May 2nd, 2019 •
1,2, 3
1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science

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In questo protocollo, introduciamo un metodo per purificare la frazione dendritica ricca di filopodia dalla struttura di protrusione simile a una coppa fagocitica sui neuroni ippocampali coltivati sfruttando l'affinità specifica e forte tra un filopopodiale dendritico molecola di adesione, TLCN, e una molecola di matrice extracellulare, vitronectina.

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Neuroscienze numero 147 filopodia dendritica sinapsi colonna vertebrale telencefalina ICAM-5 vitronectina neuroni ippocampali coltura a bassa densità microsfere magnetiche coppa fagocistica
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