Journal
/
Neuroscience
/
Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

DOI:
10.3791/63189-v

•

05:54 min

•

May 18, 2022

•

, ,
1Biology department,University of Virginia

Chapters

  • 00:04Introduction
  • 00:35Drosophila Larvae Collection
  • 01:23Culture Media and Tool Preparation
  • 02:03Dissections and Brain Cultures
  • 02:57Proliferation Assay, Brain Fixation, and Antibody Staining
  • 04:24Results: Confocal Imaging of Stained Brain Cells
  • 05:19Conclusion

Summary

Automatic Translation

Automatic Translation

A method to reactivate quiescent neural stem cells in cultured Drosophila brain explants has been established. Using this method, the role of systemic signals can be uncoupled from tissue-intrinsic signals in the regulation of neural stem cell quiescence, entry and exit.

Tags

Keywords: Neural Stem CellsDrosophilaBrain ExplantsStem Cell ReactivationExtrinsic CuesCell CycleDissectionSchneider’s Culture Medium5-ethynyl-2′-deoxyuridine (EdU)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63189?article_type=v

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology
check_url/63189?article_type=v

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells
check_url/63189?article_type=v

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
check_url/63189?article_type=v

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals
check_url/63189?article_type=v

A Computer-assisted Multi-electrode Patch-clamp System
check_url/63189?article_type=v

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
check_url/63189?article_type=v

Laser-guided Neuronal Tracing In Brain Explants
check_url/63189?article_type=v

Enumeration of Neural Stem Cells Using Clonal Assays
check_url/63189?article_type=v

Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior
check_url/63189?article_type=v

Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

Read Article