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Journal / Bioengineering / CRISPR/Cas12a Multiplex Genome Editing of…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art CRISPR
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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art CRISPR

Article DOI: 10.3791/59350-v • 10:18 min • May 28th, 2019
May 28th, 2019 •
1,2, 1, 3,4, 1, 1
1DSM Biotechnology Center, 2Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, 3BrisSynBio, University of Bristol, Life Sciences Building, 4School of Biological Sciences, University of Bristol, Life Sciences Building

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Le système CRISPR/Cas12a, combiné à un seul réseau de crRNA, permet une édition multiplexe efficace du génome de S. cerevisiae à plusieurs loci simultanément. Ceci est démontré par la construction de caroténoïdes produisant des souches de levure qui sont ensuite utilisés pour créer de l'art pixel levure.

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Bioengineering CRISPR/Cas12a CRISPR/Cpf1 CRISPR/Cas9 multiplex genome editing Saccharomyces cerevisiae levure pixel art
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