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Ex Vivo Perfusion of the Rodent Placenta
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Ex Vivo Perfusion of the Rodent Placenta

Ex Vivo Perfusion of the Rodent Placenta

DOI:
10.3791/59412-v

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06:54 min

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May 30, 2019

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, ,
1Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy,Rutgers University, 2Environmental and Occupational Health Sciences Institute

Chapters

  • 00:04Title
  • 00:40Placenta Harvest
  • 03:08Placental Perfusion
  • 04:53Sample Collection
  • 05:45Results: Representative Ex Vivo Rodent Placenta Perfusion
  • 06:34Conclusion

Summary

Automatic Translation

Automatic Translation

Here is presented a protocol of ex vivo maternal-fetal vascular perfusion to enable the administration of a test article into maternal vasculature and to evaluate placental transfer of xenobiotic particles or pharmacological agents in addition to alterations in placental physiology.

Tags

Keywords: Ex Vivo PerfusionRodent PlacentaPharmacological TrialsXenobiotic ContaminantsPlacenta Perfusion TechniqueHigh ThroughputPharmacological StudiesToxicological StudiesUterine CannulationUmbilical CannulationGestational Day 20Pregnant RatUterine ArteryUmbilical ArteryUmbilical VeinMaternal Placenta Fetal UnitAmniotic MembraneUmbilical CordUterine ArteryUterine Vein

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