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Immunology and Infection

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JoVE Journal Immunology and Infection
Skräddarsy in vivo cytotoxicitet assays att studera Immunodominans i Tumörspecifika CD8+ T cell svar
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Skräddarsy in vivo cytotoxicitet assays att studera Immunodominans i Tumörspecifika CD8+ T cell svar

Article DOI: 10.3791/59531-v • 10:13 min • May 6th, 2019
May 6th, 2019 •
1, 1, 1,2,3,4
1Department of Microbiology and Immunology, Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy, Western University, 3Department of Surgery, Division of General Surgery, Western University, 4Lawson Health Research Institute

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Vi beskriver här en flödescytometri-baserad in vivo dödande analys som möjliggör undersökning av immunodominans i cytotoxiska T-lymfocyter (CTL) svar på en modell tumör antigen. Vi ger exempel på hur denna eleganta analys kan vara anställd för mekanistiska studier och för läkemedels effekt testning.

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Immunologi och infektion CD8+ T-celler cytotoxicitet cancer immunodominans tumör-härledda peptider stora T-antigen regulatoriska t-celler PD-1 Checkpoint-hämmare läkemedels effekt testning flödescytometri
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