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Skreddersy in vivo cytotoksisitet analyser for å studere Immunodominance i tumor spesifikke CD8+ T celle svar
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
DOI:

10:13 min

May 06, 2019

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Chapters

  • 00:04Title
  • 01:14T Antigen (Ag)-Expressing Tumor Cell Inoculation
  • 02:29Target Splenocyte Preparation
  • 04:10Target Splenocyte Coating with Irrelevant and Cognate Peptides and Carboxyfluorescein Succinimidyl Ester (CFSE) Labeling
  • 05:44CFSE-Labeling Evaluation
  • 06:50CFSE-Labelled Target Cell Injection into Nave and T-Ag-Primed Recipients
  • 07:31Data Acquisition
  • 08:21Results: Representative Analyses of CD8+ T Cell-Mediated Cytotoxicity
  • 09:36Conclusion

Summary

Automatic Translation

Vi beskriver her en flyt flowcytometri-basert in vivo drap analysen som muliggjør undersøkelse av immunodominance i cytotoksisk T-lymfocytter (CTL) svar på en modell svulst antigen. Vi gir eksempler på hvordan denne elegante analysen kan være ansatt for mekanistisk studier og for narkotika effektivitet testing.

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