Journal
/
Immunology and Infection
/
活体细胞毒性试验研究肿瘤特异性 CD8+ t 细胞反应中的免疫调节
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
Please note that all translations are automatically generated. Click here for the English version.

活体细胞毒性试验研究肿瘤特异性 CD8+ t 细胞反应中的免疫调节

DOI:
10.3791/59531-v

•

10:13 min

•

May 06, 2019

•

, ,
1Department of Microbiology and Immunology,Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy,Western University, 3Department of Surgery, Division of General Surgery,Western University, 4Lawson Health Research Institute

Chapters

  • 00:04Title
  • 01:14T Antigen (Ag)-Expressing Tumor Cell Inoculation
  • 02:29Target Splenocyte Preparation
  • 04:10Target Splenocyte Coating with Irrelevant and Cognate Peptides and Carboxyfluorescein Succinimidyl Ester (CFSE) Labeling
  • 05:44CFSE-Labeling Evaluation
  • 06:50CFSE-Labelled Target Cell Injection into Nave and T-Ag-Primed Recipients
  • 07:31Data Acquisition
  • 08:21Results: Representative Analyses of CD8+ T Cell-Mediated Cytotoxicity
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

我们这里描述了一种基于流式细胞仪的体内杀伤检测方法, 该方法能够检查细胞毒性 T 淋巴细胞 (CTL) 对模型肿瘤抗原的反应中的免疫调节。我们提供了如何将这种优雅的检测方法用于机械研究和药物疗效测试的示例。

Tags

In Vivo Cytotoxicity AssayTumor-specific CD8+ T Cell ResponsesImmunodominanceTumor AntigensCytotoxic T Cell ResponsesNK CellsInnate-like T LymphocytesAdherent Tumor Cell LineTarget Splenocyte PreparationC57BL/6 Mice

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

T细胞受体在小鼠T细胞的逆转录病毒转导

竞争的原点实验研究肠嗜T细胞迁移

定量高通量单细胞的细胞毒性T细胞的测定

荧光目标阵列的T细胞反应评价中的应用<em>在体内</em

尾部皮肤来研究异基因CD4 + T细胞应答的小鼠移植

对于纳米颗粒疫苗接种后的分析抗原特异性CD8 + T细胞应答整体动物成像和流式细胞技术

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

一种研究人体 T 细胞召回反应的吸水泡协议

酶联免疫吸附法表征小鼠胸腺依赖性和胸腺独立免疫球蛋白同种反应

优化干扰素伽隆弹性体检测在几内亚猪模型接种疫苗后 t 细胞反应

Read Article