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刺激されたラマン散乱に基づく非線形顕微鏡の実施
JoVE Journal
Engineering
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JoVE Journal Engineering
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
DOI:

09:13 min

July 06, 2019

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Chapters

  • 00:04Title
  • 00:58Alignment of the Microscope Spatial Beam Overlap
  • 01:58Alignment of the Microscope Temporal Synchronization of the Beams Use of the Autocorrelator
  • 03:24Alignment of the Microscope Aligning the Femtosecond Laser Beams :OPO and Ti:Sa
  • 04:44Optimization of Temporal Synchronization of Beams
  • 05:48Optimization of Spatial Overlap of Beams – Transmission Image Acquisition of OPO and Ti:Sa
  • 06:48SRS Image Acquisition
  • 07:40Results: Nonlinear Microscope Based on Stimulated Raman Scattering
  • 08:43Conclusion

Summary

Automatic Translation

本原稿では、レーザー走査顕微鏡とのSRS実験セットアップの統合により得られる刺激ラマン散乱(SRS)顕微鏡の実装について説明する。SRS顕微鏡は2つのフェムト秒(fs)レーザー源、Ti-Sapphire(Ti:Sa)および同期光学パラメトリック発振器(OPO)に基づいている。

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