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Implantation of an Isoproterenol Mini-Pump to Induce Heart Failure in Mice
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Implantation of an Isoproterenol Mini-Pump to Induce Heart Failure in Mice

Article DOI: 10.3791/59646-v • 05:08 min • October 3rd, 2019
October 3rd, 2019 •
1, 2, 3, 4, 1, 2
1Department of Anesthesiology, University of California, 2Department of Medicine, University of California, 3Department of Microbiology, Immunology & Molecular Genetics, University of California, 4Department of Molecular, Cell, and Developmental Biology, University of California

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Summary
Automatic Translation

The chronic administration of isoproterenol via an implanted osmotic pump has been used widely to mimic advanced heart failure in mice. Here, we describe detailed methods in surgical mini-pump implantation for the continuous isoproterenol administration over 3 weeks, as well as, echocardiographic assessment for the successful model creation.

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Implantation Isoproterenol Mini-Pump Heart Failure Mice Chronic Administration Osmotic Pump Surgical Expertise LAD Model TAC Model Dr. Vincent Ren Dr. Yibin Wang's Laboratory Body Weight Concentration Isoproterenol Solution Sodium Chloride Osmotic Pump Filling Tube Syringe Plunger Pump Reservoir Flow Moderator
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