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JoVE Journal
Cancer Research

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患者生物流体中肿瘤相关循环DNA的检测与监测
 
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患者生物流体中肿瘤相关循环DNA的检测与监测

Article DOI: 10.3791/59721-v 06:53 min June 8th, 2019
June 8th, 2019

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Summary

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Click here for the English version.

在这里,我们提出一个协议,以检测肿瘤体突变在循环DNA存在于患者生物流体(生物流体)。我们的滴数字聚合酶链反应(dPCR)方法能够定量肿瘤突变等位基因频率(MAF),促进对肿瘤反应的诊断和时间监测的微创补充。

Tags

癌症研究,第148期,液体活检,数字液滴PCR,循环细胞游出DNA,血浆,血清,脑脊液
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