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JoVE Journal Genetics
Mesure de bk-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
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Mesure de bk-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

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Dans ce manuscrit, un protocole est présenté pour effectuer la mesure FACS-basée de bk-polyomavirus activité transcriptionnelle en utilisant des cellules HEK293T transfecté avec un plasmide journaliste bidirectionnel exprimant tdTomato et eGFP. Cette méthode permet en outre de déterminer quantitativement l'influence de nouveaux composés sur la transcription virale.

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Génétique Numéro 149 BK-Polyomavirus BKPyV Région de contrôle non codante NCCR activité de promoteur bidirectionnel FACS activité antivirale transplantation rénale grand antigène T SV40
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