Journal
/
Behavior
/
Comparative Lesions Analysis Through a Targeted Sequencing Approach
JoVE Journal
Behavior
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Behavior
Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

DOI:
10.3791/59844-v

•

08:16 min

•

November 05, 2019

•

, , , , , , , ,
1ARC-Net Research Centre,University and Hospital Trust of Verona, 2Department of Diagnostics and Public Health, Section of Pathology,University and Hospital Trust of Verona, 3Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit,University of Padua

Chapters

  • 00:04Title
  • 00:48Library Pooling and Sequencing Run
  • 02:28Sample Library Dilution and Loading
  • 03:00Next Generation Sequencing
  • 03:43Mutation and Copy-Number Variation (CNV) Analysis
  • 05:00Somatic Mutation Indexing
  • 05:36Results: Representative Comparative Lesion Analyses
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.

Tags

Targeted SequencingGenetic RelatednessMolecular AlterationsDisease ProgressionIntratumor HeterogeneityAnti-tumor StrategiesSolid Tumor TypesSequencing RunIon Proton SystemIon Gene Studio S5 SystemIon AmpliSeq 2.0 Library KitIon ChefCoverage Analysis PluginGRCH37HG19 GenomeBarcode SetClonal AmplificationNext Generation Sequencing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

Measurement of Fronto-limbic Activity Using an Emotional Oddball Task in Children with Familial High Risk for Schizophrenia

Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach

Pavlovian Conditioned Approach Training in Rats

Protocol for Data Collection and Analysis Applied to Automated Facial Expression Analysis Technology and Temporal Analysis for Sensory Evaluation

Treatment of Ankle Osteoarthritis with Total Ankle Replacement Through a Lateral Transfibular Approach

Use of a Wireless Video-EEG System to Monitor Epileptiform Discharges Following Lateral Fluid-Percussion Induced Traumatic Brain Injury

A Machine Learning Approach to Design an Efficient Selective Screening of Mild Cognitive Impairment

Evaluating Skilled Prehension in Mice Using an Auto-Trainer

Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse

Read Article