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Journal / Bioengineering / Humana iPSC-härledda Kardiomyocyte nätverk på…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Humana iPSC-härledda Kardiomyocyte nätverk på Multiwell Micro-elektrod arrayer för återkommande åtgärder potentiella inspelningar
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Humana iPSC-härledda Kardiomyocyte nätverk på Multiwell Micro-elektrod arrayer för återkommande åtgärder potentiella inspelningar

Article DOI: 10.3791/59906-v • 08:53 min • July 15th, 2019
July 15th, 2019 •
*1, *1, 1, 1, 1,2,3
1Aurora Research Institute, Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science, University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine, Johns Hopkins University
* These authors contributed equally

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Denna artikel innehåller en uppsättning protokoll för utveckling av Human inducerad pluripotenta stamcells-derived cellen (hipsc-cm) nätverk odlade på multispot MEA plattor för att reversibelt elektroporate cellmembranet för åtgärder potentiella mätningar. Inspelningar med hög kapacitet erhålls från samma cell platser upprepade gånger under dagar.

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Bioteknik iPSC-härledda kardiomyocyter multi-elektrod array actionpotential fältpotential kardiell elektrofysiologi elektroporation Drug screening
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