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Journal / Immunology and Infection / 체외 전사 mRNA를 가진 1 차적인 대식세포의 고효율…
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Immunology and Infection

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JoVE Journal Immunology and Infection
체외 전사 mRNA를 가진 1 차적인 대식세포의 고효율 형질감염
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체외 전사 mRNA를 가진 1 차적인 대식세포의 고효율 형질감염

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

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대식세포, 특히 1차 대식세포는 비자기 기원의 분자를 검출하는 것을 전문으로 하기 때문에 트랜스펙트에 도전하고 있습니다. 우리는 플라스미드와 같은 DNA 템플릿에서 생성된 mRNA를 가진 1 차적인 대식세포의 고도로 능률적인 형질혈을 허용하는 프로토콜을 기술합니다.

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면역학 및 감염 문제 153 원발성 대식세포 맥막 대식세포 골수 유래 대식세포 면역 세포 체외 전사 mRNA 형질감염
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