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Journal / Cancer Research / Differentiering av Musbröstepitel HC11 och EpH4…
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JoVE Journal Cancer Research
Differentiering av Musbröstepitel HC11 och EpH4 Celler
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Differentiering av Musbröstepitel HC11 och EpH4 Celler

Article DOI: 10.3791/60147-v • 09:32 min • February 27th, 2020
February 27th, 2020 •
*1,2, *1, *1,3, 1,4, 1, 1, 2, 1
1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine, Queen's University, Kingston, 2Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus, William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
* These authors contributed equally

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Vi beskriver tekniker för differentiering induktion av två bröst epitel linjer, HC11 och EpH4. Medan båda kräver fetala kalv serum, insulin och prolaktin för att producera mjölkproteiner, EpH4 celler kan helt skilja i mammografi i tredimensionell kultur. Dessa kompletterande modeller är användbara för signaltransduktionsstudier av differentiering och neoplasi.

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Cancerforskning Utgåva 156 celldifferentiering bröstepitelceller prolaktin EHS-matris
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