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Journal / Bioengineering / Screening och identifiering av små peptider…
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Bioengineering

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JoVE Journal Bioengineering
Screening och identifiering av små peptider riktade fibroblast tillväxtfaktor Receptor2 använda en phage display peptid bibliotek
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Screening och identifiering av små peptider riktade fibroblast tillväxtfaktor Receptor2 använda en phage display peptid bibliotek

Article DOI: 10.3791/60189-v • 07:32 min • September 30th, 2019
September 30th, 2019 •
*1, *1, 1, 1
1Institute of Biomedicine & Department of Cell Biology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine
* These authors contributed equally

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Häri presenterar vi ett detaljerat protokoll för screening av små peptider som binder till FGFR2 med hjälp av en fagdisplay peptid bibliotek. Vi analyserar vidare Affiniteten hos de utvalda peptiderna mot FGFR2 in vitro och dess förmåga att undertrycka cellproliferation.

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Bioteknik liten peptid fagdisplay peptid bibliotek titerbestämning plack amplifiering FGFR2 affinitet
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