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Trækkraft mikroskopi integreret med Microfluidics til kemo-aktisk kollektiv migration
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
DOI:

10:53 min

October 13, 2019

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Chapters

  • 00:04Title
  • 00:47Fabrication of Polydimethylsiloxane (PDMS) Stencil and Microchannel
  • 02:39Preparation of Bottom Glass with Polyacrylamide (PA) Gel
  • 04:41Micropatterning of Cell Islands and Assembly of PA Gel with PDMS Microchannel
  • 06:14Integrated Microfluidic System
  • 07:32Image Acquisition
  • 08:27Results: Mechanical Interactions of MDCK Cell Islands
  • 09:55Conclusion

Summary

Automatic Translation

Kollektiv celle migration i udvikling, sårheling, og kræft metastaser er ofte styret af gradienter af vækstfaktorer eller signalering molekyler. Beskrevet her er et eksperimentelt system, der kombinerer trækkraft mikroskopi med et mikrofluidisk-system og en demonstration af, hvordan man kvantificerer mekanik af kollektiv migration under biokemisk gradient.

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