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Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
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Bioengineering
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JoVE Journal Bioengineering
Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

DOI:
10.3791/60383-v

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08:10 min

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October 06, 2019

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,
1Department of Biomedical Engineering,University of Delaware, 2Department of Materials Science & Engineering,University of Delaware, 3Delaware Biotechnology Institute

Chapters

  • 00:04Title
  • 00:40Base Hydrogel Photopolymerization
  • 02:04Binary Image to Digital Mask Conversion
  • 03:02Base Hydrogel Soak
  • 03:44Microscope Configuration
  • 05:15Fiducial Marker Array Photopatterning
  • 06:01Fiducial Marker and Cell Imaging
  • 06:31Results: Representative Reference-Free Traction Force Microscopy
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force measurements.

Tags

Keywords: Traction Force MicroscopyReference-freePhotopolymerizationHydrogelPDMSCoverslipPetri DishPBSNVPMATLABBinary ImageDigital Mask

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