Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
 

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Article DOI: 10.3791/60383-v
October 6th, 2019

Chapters

Summary October 6th, 2019

This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force measurements.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter