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测定细菌和化学物质对
开牛结
不二病的肠道渗透性的影响
JoVE Journal
Medicine
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JoVE Journal
Medicine
Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
测定细菌和化学物质对
开牛结
不二病的肠道渗透性的影响
DOI:
10.3791/60419-v
•
07:23 min
•
December 03, 2019
•
Tram Anh Ngoc Le
,
Baskar Selvaraj
2
,
Jae Wook Lee
2
,
Kyungsu Kang
2
1
Gangneung Institute of Natural Products
,
Korea Institute of Science and Technology
,
2
Division of Bio-Medical Science & Technology
,
Korea University of Science and Technology (UST)
Chapters
00:05
Title
01:12
Preparation of
P. aeruginosa
PAO1 Culture
01:58
Preparation of Nematode Growth Medium (NGM) Plates for Testing the Effects of Bacteria and DIM on the Intestinal Permeability of
C. elegans
02:57
Bacteria or DIM Treatment and FITC-Dextran Feeding
04:19
Imaging of
C. elegans
with the Operetta Imaging System and Determination of Intestinal Permeability
05:43
Results: Effects of Bacteria on Intestinal Permeability of
P. aeruginosa
06:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何测量
卡内沙布迪炎的
肠道渗透性。该方法有助于对肠道健康进行与肠道细菌与其宿主相互作用相关的基础生物学研究,并有助于筛选出益生菌和化学制剂,以治疗漏肠综合征和炎症性肠病。
Tags
Caenorhabditis Elegans
Intestinal Permeability
Bacteria
Chemicals
High-throughput Imaging
Leaky Gut Syndrome
Inflammatory Bowel Diseases
P. Aeruginosa
E. Coli
NGM Plates
DIM Plates
DMSO Plates
S-buffer
Article
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