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In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
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In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Article DOI: 10.3791/60427-v • 08:19 min • June 22nd, 2020
June 22nd, 2020 •
*1, *1, 1, 1, 1, 2, 1
1Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 2Department of Physiology, Kilimanjaro Christian Medical University College
* These authors contributed equally

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Summary
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This protocol aims to describe step-by-step the technique of extraction and assessment of cardiac function using skinned cardiomyocytes. This methodology allows measurement and acutemodulation of myofilament function using small frozen biopsies that can be collected from different cardiac locations, from mice to men.

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In Vitro Assessment Cardiac Function Skinned Cardiomyocytes Pathophysiology Correlation In Vivo Parameters Animal Models Humans Advantage Deep Myofilament Function Small Biopsies Frozen Samples Therapeutic Interventions Experiment Cardiomyocyte Extraction Select Glue Activate Size Striation Shape Temperature Testing Apparatus Force Transducer Motor Myocardial Sample Petri Dish Relax ISO Solution Scalpel Tissue Pieces Cell Damage Cut Pipette Tip Solution And Tissue Fragments Potter-Elvehjem Glass Grinder Mechanically Disrupt Tissue
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