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Quantification des cellules proliférantes et mortes chez les entéroïdes
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Quantification des cellules proliférantes et mortes chez les entéroïdes

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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Le protocole présenté utilise la cytométrie de flux pour quantifier le nombre de cellules proliférantes et mortes dans les entéroïdes de souris cultivés. Cette méthode est utile pour évaluer les effets du traitement médicamenteux sur la prolifération et la survie des organoïdes.

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Biologie Numéro 155 entéroïdes intestin cytométrie du débit prolifération EdU propidium iodure
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