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December 20, 2019
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Presented here is a protocol for the isolation of mouse hepatocytes from adult mouse livers using a modified collagenase perfusion technique. Also described is the long-term culture of hepatocytes in a 3D collagen sandwich setting as well as immunolabeling of the cytoskeletal components to study bile canalicular formation and its response to treatment.
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Cite this Article
Korelova, K., Jirouskova, M., Sarnova, L., Gregor, M. Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro. J. Vis. Exp. (154), e60507, doi:10.3791/60507 (2019).
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