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Medicine
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研究活体器官的肠道屏障分解
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Investigating Intestinal Barrier Breakdown in Living Organoids
Please note that all translations are automatically generated.
Click here for the English version.
研究活体器官的肠道屏障分解
DOI:
10.3791/60546-v
•
07:18 min
•
March 26, 2020
•
Marco Bardenbacher
,
Barbara Ruder
,
Natalie Britzen-Laurent
,
Elisabeth Naschberger
,
Christoph Becker
,
Ralph Palmisano
,
Michael Stürzl*
1
,
Philipp Tripal*
1,3
1
Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center
,
Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen
,
2
Department of Medicine 1
,
Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen
,
3
Optical Imaging Centre Erlangen (OICE)
,
Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg
Chapters
00:05
Introduction
00:57
Barrier Integrity Measurement Preparation
03:04
Organoid Permeability Assay
05:39
Results: Representative Intestinal Organoid Barrier Integrity Analysis
06:32
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种量化小肠道器官的屏障完整性的技术。该方法以活器官为基础,能够以时间解析的方式对不同的屏障完整性调节物质或其组合进行顺序调查。
Tags
Intestinal Barrier
Organoids
Barrier Integrity
Tight Junction Proteins
Epithelial Barrier
Cell Culture
Permeability Assay
Interferon Gamma
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