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NF-κB-afhængig luciferase aktivering og kvantificering af genekspression i salmonella inficerede vævskultur celler
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
DOI:

10:57 min

January 12, 2020

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Chapters

  • 00:04Title
  • 01:13Cell Passaging and Seeding
  • 02:51Preparation of Bacteria
  • 06:23Infection of Cells
  • 08:00Luciferase Analysis
  • 09:18Results: Luminescence Assay and RT-qPCR Analysis
  • 10:10Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol til hurtigt og nemt at måle nuklear faktor Kappa-lys-kæde forstærker af aktiverede B-celler (NF-κB) aktivering i cellelinjer, der udtrykker NF-κB:: luciferase reporter konstruerer, via målinger af luminescens i celle lysatet. Desuden bestemmes genekspression via RT-qPCR isoleret fra celler inficeret med salmonella typhimurium.

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