/

Immunology and Infection

/

Multi-scale Analysis of Bacterial Growth Under Stress Treatments

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Multi-scale Analysis of Bacterial Growth Under Stress Treatments

Multi-scale Analysis of Bacterial Growth Under Stress Treatments

DOI:

10.3791/60576-v

•

12:08 min

•

November 28, 2019

•

Julien Cayron, Christian Lesterlin

¹Molecular Microbiology and Structural Biochemistry (MMSB),University of Lyon 1, CNRS, Inserm

Chapters

00:04Title
01:03Cell Culture, Stress-induction, and Sampling Procedure
02:40Plating Assay and Flow Cytometry
04:55Snapshot Microscopy Imaging
05:49Microfluidics Time-lapse Microscopy Imaging
08:19Image Analysis
09:38Results: Flow Cytometry and Microscopy Analysis
11:31Conclusion

Summary

Automatic Translation

This protocol allows a time-resolved description of bacterial growth under stress conditions at the single-cell and the cell population levels.

Tags

Keywords: Bacterial Growth Stress Treatment Multi-scale Analysis Cell Viability Morphology DNA Content Population Recovery Pharmaceutical Industry Antimicrobial Screening Dose-dependent Time-lapse Microscopy Dilution And Plating Assay Flow Cytometry Microscopy Snapshot Cephalexin

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Introducing Shear Stress in the Study of Bacterial Adhesion

Growth of Mycobacterium tuberculosis Biofilms

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Read Article